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All Journal AQUATIC SCIENCE & MANAGEMENT e-Journal BUDIDAYA PERAIRAN JURNAL PESISIR DAN LAUT TROPIS JURNAL PERIKANAN DAN KELAUTAN TROPIS JURNAL LPPM BIDANG SAINS DAN TEKNOLOGI
Veibe Warouw
Fakultas Perikanan dan Ilmu Kelautan, Universitas Sam Ratulangi, Manado, Sulawesi Utara
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science

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Journal : JURNAL PESISIR DAN LAUT TROPIS

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KAJIAN KESESUAIAN LAHAN EKOWISATA MANGROVE DIMENSI EKOLOGI ( KASUS PADA PULAU BUNAKEN BAGIAN TIMUR, KELURAHAN ALUNG BANUA, KECAMATAN BUNAKEN KEPULAUAN, KOTA MANADO) Bacmid, Kiki Nadila; Schaduw, Joshian N W; Warouw, Veibe; Darwisito, Surya; Kaligis, Erly Y; Wantasen, Adnan
JURNAL PESISIR DAN LAUT TROPIS Vol 7, No 3 (2019): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.7.3.2019.24257

Abstract

Ecotourism as a form of tourism that emphasizes responsibility for nature conservation, provides economic benefits and maintains cultural needs for the local community. Mangrove forests with their uniqueness are natural resources that have the potential to be used as tourist attractions. The purpose of this study was to determine community structure, mangrove canopy cover and suitability of mangrove ecotourism land. This study used the line transect method for mangrove community structure, and hemispherical photography for the percentage of mangrove cover. The results of the study of the mangrove community structure were the highest density value, namely at station 1 on the transect 2 types of S.alba 0.16 ind / m2 relative 88.89% and the lowest density value in the type R. mucronata and R.apiculata 0.01 ind / m2 relatively 5.56% on transect 2 at station 1. The highest frequency type value at station 1 on the transect 1 type S. alba which is 1.00 relative 100% and the lowest frequency at station 1 transect 2 types R, piculate which is relative 0.50 33.33. The highest closing value of type on transect 2 standard 1 type S. alba 10.26 relative value 92.63%. Whereas the lowest type 3 transect closure is type R. piculate 0.49 the relative is 7.86%. The highest Important Value Index is at station 1 on transect 1, namely type S. alba 300 and lowest at station 1 on transect 2 types R. mucronata 30.55. The highest diversity value on transect 2, 0.43. Evenness index value (E) at station 1 on transect 1 is 0 and transect 2 is 0.39. While station 2 on transect 1 0 and transect 2 0.51. regarding the criteria for mangrove damage, at 2 stations included in the rare category with inya 50% canopy cover. The overall results of land suitability for coastal tourism in the Bunaken Island mangrove tourism category fall into the very appropriate category.Keywords: Mangrove, Ecotourism, Bunaken IslandEkowisata sebagai suatu bentuk wisata yang menekankan tanggung jawab terhadap kelestarian alam, memberikan manfaat secara ekonomi dan mempertahankan kebutuhan budaya bagi masyarakat setempat. Hutan mangrove dengan keunikan yang dimilikinya, merupakan sumberdaya alam yang sangat berpotensi untuk dijadikan sebagai tempat kunjungan wisata. Tujuan dari penelitian ini adalah untuk mengetahui struktur komunitas, tutupan kanopi mangrove dan kesesuaian lahan ekowisata mangrove. Penelitian ini menggunakan metode line transek terhadap struktur komunitas mangrove, dan hemispherical photography untuk persentase tutupan mangrove. Hasil penelitian struktur komonitas mangrove yaitu Nilai kerapatan tertinggi yaitu pada stasiun 1 di transek 2 jenis S.alba  0.16 ind/m2 relatifnya 88.89% dan nilai kerapatan terendah pada jenis R. mucronata dan R.apiculata 0.01 ind/m2 relatifnya 5.56% di transek 2 pada stasiun 1 .Nilai frekuensi jenis tertinggi pada stasiun 1 di transek 1 jenis S.alba yaitu 1.00 relatifnya 100% dan frekuensi terendah pada stasiun 1 transek 2 jenis R, piculate yaitu  0.50 relatifnya  33.33. Nilai penutupan jenis tertinggi pada transek 2 stasun 1 jenis S. alba 10.26 nilai relatifnya 92.63 %. Sedangkan penutupan jenis terendah transek 3 jenisR.  piculate  0.49 relatifnya 7.86%. Indeks Nilai Penting tertinggi yaitu pada stasiun 1 di transek 1 yaitu jenis S.alba 300 dan terendah pada stasiun 1 di transek 2 jenis R.mucronata 30.55.Nilai keanekaragaman tertinggi pada transek 2, 0.43. Nilai indeks kemerataan (E) pada stasiun 1 di transek 1 yaitu 0 dan transek 2 0,39.  Sedangkan stasiun 2 pada transek 1 0 dan transek 2 0.51. tentang kriteria kerusakan mangrove, pada ke 2 stasiun termasuk pada kategori jarang dengan tutupan kanopinya ≤ 50% . Hasil keseluruhan kesesuaian lahan untuk wisata pantai kategori wisata mangrove Pulau Bunaken masuk pada kategori sangat sesuai.Kata kunci: Mangrove, Ekowisata, Pulau Bunaken
KOMUNITAS ASCIDIA DI PESISIR MALALAYANG DUA, TELUK MANADO, SULAWESI UTARA Malintoi, Adrianus; Rumengan, Inneke F M; Roeroe, Kakaskasen A; Warouw, Veibe; Rondonuwu, Ari B; Ompi, Medy
JURNAL PESISIR DAN LAUT TROPIS Vol 8, No 1 (2020): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.8.1.2020.27403

Abstract

Field survey on ascidian community was conducted along the coastal area of Malalayang Dua in order to find out species of ascidia, species abundance, and ascidian substrates. A survey method and quadrant transects were applied. Pictures were taken, while species and their substrates were sampled.  Species identification was based on morphological characteristics, while substrate type identification was based on ascidian species attachment.  The results shows that 21 ascidian species were found in the the coastal of Malalayang Dua.  Didemnum molle was the highest abundant species in the area, followed by Polycarpa aurata, Polycarpa sp.4. and Polycarpa sp.2.. Dead coral algaes (DCA) were found to be the most preferred  substrates by ascidians in the area. Keywords : ascidia, species, substrate, distribution, and abundance Survei lapangan terhadap komunitas ascidia dilakukan  di pesisir Malalayang Dua untuk mendapatkan data jenis, kelimpahan, dan substrat ascidia.  Metode yang digunakan yaitu metode survei jelajah dan transek kuadran. Identifikasi jenis ascidia dilakukan berdasarkan karakteristik morfologi.  Hasil penelitian ditemukan ada 21 jenis ascidia.  Substrat jenis death coral algae (DCA) merupakan substrat yang paling banyak ditempati ascidia. Kelimpahan ascidia tertinggi adalah Didemnum molle di pesisir Malalayang Dua, diikuti oleh Polycarpa aurata,   Polycarpa sp.4. dan Polycarpa sp.2. Death coral alga (DCA) ditemukan sebagai substrat yang paling disukai oleh ascidia di daerah itu. Kata Kunci : ascidia, spesies, substrat, distribusi, dan kelimpahan  
Penapisan (skrining) aktivitas antibakteri beberapa ekstrak spons dari Teluk Manado Nowin, Edgar; Warouw, Veibe; Rimper, Joice; Paulus, James; Pangkey, Henneke; Sumilat, Deiske
JURNAL PESISIR DAN LAUT TROPIS Vol 6, No 1 (2018): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.6.1.2018.20198

Abstract

Spons merupakan salah satu biota laut yang sangat prospektif sebagai sumber senyawa bahan-bahan alami seperti peptida, terpenoid, steroid, asetogenin, alkaloid, halida siklik, dan senyawa nitrogen lainnya. Senyawa-senyawa ini memiliki aktivitas farmakologis seperti antifouling, antitumor, antiinflamasi, antivirus, antibakteri, antijamur, antimalaria. Telah diekstrak dua belas spons yang dikoleksi dari Teluk Manado, Sulawesi Utara. Pengujian aktivitas antibakteri dalam penelitian ini menggunakan metode Kirby-Bauer yang dimodifikasi. Hasil yang diperoleh dalam penelitian ini  dari  koleksi beberapa ekstrak spons sebanyak 9,442 gram melalui proses maserasi dengan pelarut etanol dan evaporasi.  Kedua belas ekstrak yang diskrining didapatkan 10,6 mm (ekstrak 1), 7,4 mm (ekstrak 4) dan (10,6 mm) ekstrak 9  yang memiliki aktivitas antibakteri terhadap bakteri Bacillus megaterium sedangkan pada media bakteri Escherichia coli hanya ekstrak 1 (8,9 mm) yang dapat menghambat pertumbuhan bakteri. Kontrol positif sebagai pembanding memiliki aktivitas yang jauh lebih besar terhadap bakteri B. megaterium dan E.  coli sehingga senyawa antibakteri yang terdapat dalam ekstrak spons yang dikoleksi dari Teluk Manado digolongkan sebagai senyawa yang bersifat sedang/moderate.
KARAKTERISTIK MORFOLOGI BAKTERI SIMBION SPONS MENYERUPAI Cribochalina sp DARI PERAIRAN MALALAYANG SULAWESI UTARA Poluan, Gledys Giacinta; Ginting, Elvy Like; Wullur, Stenly; warouw, Veibe; Losung, Fitje Vera; Salaki, Meiske
JURNAL PESISIR DAN LAUT TROPIS Vol 7, No 3 (2019): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.7.3.2019.24452

Abstract

Bacteria are found to be highly associated with various marine organisms, such as sponges. Sponges are known as the product of bioactive compounds. However, some of the compounds produced by sponges are obtained by the simbion-bacteria. Therefore this situation enables sponges simbion bacteria to play a major role in producing bioactive compounds that have been isolated from the sponge. The purpose of this study is to isolate and determine the characteristics of morphology of spongy simbion bacteria resembling Cribochalina sp, which was taken from Malalayang waters, North Sulawesi. Sponge simbion bacteria grow on NB media. Initially, a free-breeding colony was being done before bacteria were accrued in the NA media by 2% sponge broth (patent SID201906301) by means of Strike Plate methods. Based on to this study we had isolate five spongy simbion bacteria that resembling the Cribochalina sp. All five of these isolations have different characteristics of morphology in terms of color, shape, size, and elevation.      Keywords : bacteria, isolation, simbionts, sponges 
ISOLASI PIGMEN KAROTENOID PADA KEPITING Metopograpsus sp. BETINA Manik, Franciskus K.I.; Paransa, Darus J; Mantiri, Desy MH; Ginting, Elvy L; Warouw, Veibe; Moningkey, Rudy
JURNAL PESISIR DAN LAUT TROPIS Vol 8, No 1 (2020): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.8.1.2020.27494

Abstract

Metopograpsus sp is one of species of crabs usually found in indo-pacifik and lived at mangrove's trunk. The characteristic of this type of crab has type color on the carapace organ that indicate the presence of carotenoid. carotenoid pigments are natural dyes in plants and crustacea. The purpose of this study is to determined type of carotenoid pigments of metopograpsus sp by using TLC Method. The result of this study were identified three type of pigments of metopograpsus sp that are β-Karoten, Astaxanthin, dan Astacene.Key word : Metopograpsus sp, TLC, Carotenoid pigments.AbstrakMetopograpsus sp adalah kepiting mangrove yang biasanya ditemukan di Indo-Pasifik dan hidup dibatang pohon mangrove. Ciri khas dari kepiting ini memiliki corak berwarna pada karapasnya sehingga diasumsikan kepiting tersebut mengandung pigmen karotenoid. pigmen karatenoid adalah pewarna alami yang terdapat pada tanaman dan hewan krustasea. Tujuan dari penelitian ini untuk mengetahui jenis-jenis pigmen karotenoid pada kepiting Metopograpsus sp dengan menggunakan metode KLT. Hasil penelitian terdapat tiga jenis pigmen karotenoid yang teridentifikasi pada kepiting Metopograpsus sp yaitu : β-Karoten, Astaxanthin, dan Astacene.Kata Kunci : Metopograpsus sp, KLT, pigmen karotenoid
PENENTUAN STRUKTUR MOLEKUL KOLAGEN SISIK IKAN KAKATUA (Scarus sp) BERDASARKAN SERAPAN MOLEKUL TERHADAP GELOMBANG FTIR (FOURIER-TRANSFORM INFRARED SPECTROSCOPY ANALYSIS) Mberato, Shellyn Prastisia; Rumengan, Inneke F M; Warouw, Veibe; Wullur, Stenly; Rumampuk, Natalie D T; Undap, Suzanne L; Suptijah, Pipih; Luntungan, Aldian Hein
JURNAL PESISIR DAN LAUT TROPIS Vol 8, No 1 (2020): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.8.1.2020.27285

Abstract

 Parrot fish (Scarus sp) is a commodity which commonly consumed in North Sulawesi. High consumption of this fish has caused the high amount of fish scales as wastes. As parrot fish scales contain protein that could be transformed into commercial products such as collagen. Collagen could be applied in the industrial fields including cosmetics and pharmaceutics.  The purpose of this study was to determine molecular structure of collagen derived from the wet and dry parrot fish (Scarus sp) scales, based on molecular absorption of electromagnetic in the infrared region of the fourier transform infrared spectroscopy.Preparation of collagen of fish scales both in wet and dry forms, was initially performed with pre-treatment of raw materials by maceration in sodium hydroxide (NaOH) solution for 48 hours. Then hydrolysis process was conducted in hydrochloric acid (HCl) solution again for 48 hours to remove mineral contents of the scales.  Collagen yield of fish scales in wet and dry forms was 2.23% and 3.00%, respectively, with pH 7, and the respective  water content was  13% and 12%. For collagen derived from the wet scales, the functional groups of amide A and B absorb the electromagnetic at infrared region of 3429 cm-1 and 2930 cm-1), respectively. Also amide I, II and III absorb the electromagnetic at infrared region of 1657 cm-1, 1452 cm-1 and 1242 cm-1, respectively. It was comparable to that of collagen derived from the dry scales, the functional groups of amide A and B absorb the electromagnetic at infrared region of 3425 cm-1 and 2910 cm-1), respectively. Also amide I, II and III absorb the electromagnetic at infrared region of 1653 cm-1, 1402 cm-1 and 1244 cm-1, respectively.  The amide  III group of  the wet scales derived collagen as well as the dry scales derive collagen absorb the electromagnetics at infrared region in the range of 1309-1229 cm-1 indicating that the fish scale derived collagen has not denatured yet, but still in triple helix structure. Molecular functional groups detected for the parrot fish scales derived collagen are in the range of those for  collagen standard.Keywords : fish scale, Scarus sp, collagen, molecule structure, proximate  AbstrakIkan kakatua (Scarus sp) merupakan salah satu jenis komiditi ikan yang banyak dikonsumsi di Sulawesi Utara. Tingginya konsumsi ikan kakatua berakibat banyaknya limbah kuliner ikan ini berupa sisik ikan. Padahal sisik ikan kakatua mengandung protein yang dapat ditransformasikan menjadi produk samping komersial seperti kolagen. Kolagen dapat diaplikasikan pada bidang industry kosmetik dan farmasika. Tujuan penelitian ini menentukan struktur molekul kolagen dari sisik ikan kakatua (Scarus sp) berdasarkan wilayah serapan gelombang infra red.Preparasi kolagen dari sisik ikan baik dalam bentuk basah maupun kering,  diawali dengan proses pre-treatment bahan baku dengan melakukan perendaman menggunakan larutan NaOH selama 48 jam. Selanjutnya adalah tahap hidrolisis yang dilakukan dengan perendaman sampel menggunakan larutan asam klorida (HCl) selama 48 jam untuk menghilangkan mineral yang ada dalam sisik. Kolagen sisik basah dan sisik kering dari ikan kakatua memiliki nilai rendemen masing-masing sebesar 2.23% dan 3.00%, nilai pH 7 serta kadar air sebesar 13% dan 12%. Pada kolagen sisik basah terdeteksi Amida A mempunyai bilangan gel (3429 cm-1), Amida B (2930 cm-1). Amida I (1657 cm-1), Amida II (1452 cm-1 ) dan Amida III (1242 cm-1), sedangkan pada kolagen sisik kering  terdeteksi Amida A mempunyai bilangan gel (3425 cm -1 ), Amida B (2910 cm-1 ). Amida I (1653 cm-1 ), Amida II (1402 cm-1 ) dan Amida III (1244 cm-1). Amida III pada kolagen sisik basah dan kolagen sisik kering terdeteksi pada wilayah serapan 1309-1229 cm-1 hal menandakan bahwa kolagen sisik  ikan kakatua belum terdenaturasi karena masih terdapat struktur triple helix. Gugus fungsional kolagen sisik kering dan kolagen sisik basah dari ikan kakatua memenuhi standar gugus fungsional kolagen standar.Kata kunci : sisik, Scarus sp, kolagen, gugus fungsi, proksimat
AKTIVITAS ANTIBAKTERI DAN ANTI-UV BEBERAPA ASCIDIAN DARI PERAIRAN PANGALISANG BUNAKEN Macpal, Yuliana; Warouw, Veibe; Sumilat, Deiske A; Paulus, James J.H.; Rumampuk, Natalie D.C.; Kreckhoff, Reni L.
JURNAL PESISIR DAN LAUT TROPIS Vol 7, No 3 (2019): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.7.3.2019.26019

Abstract

Ascidians are sesile marine invertebrates that have bioactive compounds such as antibacterial and anti-UV. The purpose of this study is to determine the antibacterial activity against Escherichia coli and Staphylococcus aureus bacteria and to test the anti-UV activity of the ascidian water fraction. Antibacterial activity test was carried out using the diffusion method (disc diffusion Kirby & Bauer) and the water fraction of ascidian that showing antibacterial activity tested in a UV spectrophotometer to see the anti-UV activity. Results of the study were obtained 4 types of ascidians extracted from partitioned into water fraction, n-hexane fraction, methanol fraction. All three fractions were tested for antibacterial activity and the result showed that there were antibacterial activities of ascidian extract Clavelina sp. against both test bacteria with inhibition of S. aureus and E. coli with strong categories. Ascidian Phlebobranch sp. showed the presence of antibacterial activity with inhibition of S. aureus and E. coli with very strong categories (16,6 mm). extract Eudistoma sp. showed the presence of antibacterial activity with inhibition S. aureus and E. coli in the medium category (9 mm). The water fraction found active in antibacterial testing is then tested using a UV spectrophotometer for anti UV testing, the result show that water fraction of the four ascidians can absorb UV-B (290-320nm) and UV-A ( 320-400nm).Keywords : Ascidian, Antibacterial, Partition, Anti- UV.
UJI ANTIBAKTERI EKSTRAK DAN FRAKSI SPONS TERHADAP Escherichia coli DAN Staphylococcus aureus SERTA POTENSINYA TERHADAP AKTIVITAS ANTI-UV Gabrielle, Monika; Sumilat, Deiske A; Warouw, Veibe; Mangindaan, Remy EP; Sinjal, Chatrien AC; Longdong, Sammy NJ
JURNAL PESISIR DAN LAUT TROPIS Vol 7, No 3 (2019): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.7.3.2019.24459

Abstract

Sponge organisms produce bioactive compounds that are toxic as a means of self-defense. The compound is known to have the potential as an antibacterial and anti-UV which can absorb sunlight with the potential to be used as a material for making sunscreen. The purpose of this study was to obtain antibacterial activity against Escherichia coli and Staphylococcus aureus bacteria and anti-UV activity from crude extracts and sponges fractions. The antibacterial test done by agar diffusion method (Kirby and Bauer diffusion disc) and the crude extract and the active fraction of antibacterial compounds were tested in a UV spectrophotometer to see its anti-UV activity. As a result, 4 species of sponges were extracted and partitioned into water fractions, methanol fractions, and n-hexane fractions. All samples were tested for antibacterial activity and the results showed antibacterial activity against S. aureus by Plakortis sp. in crude extract (9 mm) and water fraction (8.6 mm), Agelas sp. in crude extract (7 mm) and in E. coli bacteria shown by Plakortis sp. in crude extract (12.6 mm) and water fraction (9 mm), Liosina sp. in the water fraction (7.6 mm), Haliclona sp. in the water fraction (8 mm) and Agelassp. in crude extract (10.3 mm). Crude extracts and water fractions were tested using a UV spectrophotometer for anti-UV testing, the results showed that crude extract and all the water fractions of four species sponge could absorb UV-B (λ 290-320 nm) and UV-A (λ 320-400 nm).Keywords: sponges, antibacterial, partition, anti-UV
ISOLASI PIGMEN KAROTENOID DARI KEPITING Grapsus sp. (CAROTENOID PIGMENTS ISOLATION FROM CRABS Grapsus sp.) Diasasthisa, Dias; Paransa, Darus J; Mantiri, Desy MH; Rumengan, Antonius; Warouw, Veibe; Salaki, Meiske
JURNAL PESISIR DAN LAUT TROPIS Vol 7, No 2 (2019): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.7.2.2019.24216

Abstract

Carotenoids are pigments with a range of red, orange and yellow colors. In carotenoid and chlorophyll plants are located in chloroplasts which undergo photosynthesis as well as photosynthetic bacteria and fungi. Carotenoid compounds have antioxidant activity, anticancer, as precursors of vitamin A and can enhance immunity. Crustaceans like crabs Grapsus sp. have carotenoid pigments, visible red, orange, and yellow are scattered in the carapace. Pigments found in crustaceans as well as crabs of Grapsus sp. generally sourced from food eaten by the crab. To determine the process of carotenoid pigment metabolism, each crab organ of Grapsus sp. isolated using thin layer chromatography separation method and column chromatography to determine the type of pigment contained therein. Pigment types identified in the crabs of Grapsus sp. males using the TLC method: β-carotene, Astasen type, Cantasantin, Astasantin, and Adonirubin. Pigment types identified in the crabs of Grapsus sp. males using the CC method: β-carotene, Astasen, β-cryptosanthine, Zeaxantine, and Cryptosanthine. Keywords : Grapsus sp., Thin Layer Chromatography, Column Chromatography, Carotenoids Pigments.             Karotenoid merupakan pigmen dengan kisaran warna merah, orange dan kuning. Pada tumbuhan karotenoid dan klorofil terletak pada kloroplas yang mengalami proses fotosintesis seperti juga pada bakteri fotosintetik dan fungi. Senyawa karotenoid memiliki aktivitas antioksidan, antikanker, sebagai prekursor vitamin A dan dapat meningkatkan imunitas. Krustasea seperti kepiting Grapsus sp. mempunyai pigmen karotenoid, terlihat warna merah, jingga, dan kuning yang tersebar pada karapas. Pigmen yang terdapat pada krustasea demikian juga pada kepiting Grapsus sp. umumnya bersumber dari makanan yang dimakan oleh kepiting tersebut. Untuk mengetahui proses metabolisme pigmen karotenoid maka masing-masing organ kepiting Grapsus sp. diisolasi dengan menggunakan metode pemisahan kromatografi lapis tipis dan kromatografi kolom untuk mengetahui jenis pigmen yang terkandung didalamnya. Jenis pigmen yang teridentifikasi pada kepiting Grapsus sp. jantan dengan metode KLT yaitu : β- karoten, Tipe Astasen, Kantasantin, Astasantin, dan Adonirubin. Jenis pigmen yang teridentifikasi pada kepiting Grapsus sp. jantan dengan metode KK  yaitu : β- karoten, Astasen, β-kriptosantin, Zeaxantin, dan Kriptosantin. Kata Kunci : Grapsus sp., Kromatografi Lapis Tipis, Kromatografi Kolom, Pigmen Karotenoid.  
Bioprospeksi antibakteri beberapa jenis spons dari Perairan Pangalisang Bunaken Liem, Jeksen; Bara, Robert; Sumilat, Deiske; Warouw, Veibe; Losung, Fitje; Wantasen, Adnan
JURNAL PESISIR DAN LAUT TROPIS Vol 7, No 1 (2019): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.7.1.2019.22813

Abstract

Indonesia merupakan negara kepulauan terbesar di dunia yang mempunyai panjang pantai 99.093 km2 yang kaya akan terumbu karang dan biota laut lainnya. Salah satu biota yang banyak diteliti adalah spons.  Wilayah laut Indonesia merupakan salah satu pusat penyebaran terbesar spons di dunia dan diperkirakan terdapat sekitar 830 spesies yang hidup tersebar di wilayah ini. Sampel penelitian yaitu beberapa jenis spons yang diambil dari Perairan Pangalisang Bunaken. Metode yang digunakan pada penelitian ini untuk pengujian aktivitas antibakteri dari ekstrak sampel spons adalah metode difusi agar. Hasil pengujian aktivitas antibakteri dari kelima ekstraksi memperlihatkan hanya ekstrak A.chromis dan C.aerizusa yang aktif terhadap bakteri B.megaterium dengan nilai hambat rata-rata sebesar  17,6 mm dan 15,0 mm, sedangkan pada bakteri E.coli hanya ekstrak A.chromis yang memiliki aktivitas antibakteri dengan nilai rata-rata 15,0 mm. Kesimpulan dari penelitian ini, telah dilakukan ekstraksi 5 spons yaitu, Liosina paradoxa, Theonella sp, Aoptos chromis, Callyspongia aerizusa dan Haliclona sp. menggunakan metode maserasi yakni etanol. Dari 5 spons yang berhasil di uji aktivitas antibakteri hanya ekstrak A.chromis dan ekstrak C.aerizusa yang menunjukan aktivitas daya hambat terhadap bakteri B.megaterium, sedangkan pada bakteri E.coli hanya ekstrak A.chromis yang memiliki zona hambat dari kelima ekstrak yang di uji.
Co-Authors Adnan Wantasen Alfret Luasunaung Antonius Rumengan Ari B Rondonuwu, Ari B Ari Rondonuwu Bacmid, Kiki Nadila Billy Theodorus Wagey Carolus Paulus Paruntu Cyska Lumenta Dalenoh, Oktavianus Darwisito, Surya Deiske Adeliene Sumilat, Deiske Adeliene Diasasthisa, Dias Elvy L Ginting Erly Y Kaligis Farnis B. Boneka Farnis Boneka Ferdinand Frans Tilaar Fitje F. Losung, Fitje F. Fitje Losung Gabrielle, Monika Ginting, Elvy Ginting, Elvy Like Henky Manoppo Henneke Pangkey Hubu, Herlin S Inneke F. M Rumengan Iskandar, Angelitha O.T Jacobs, Reinol Janny D Kusen Janny D. Kusen Johnny Budiman Joice R.T.S.L Rimper Joshian N.W. Schaduw Kreckhoff, Reni L. Kusen, Janny Kusumaningrum, Arianda Lasut, Astrid Y. Liem, Jeksen Lindon R Pane Lohoo, Anneke V Longdong, Sammy NJ Losung, Fitje Vera Lumenta, Cysca Lumingas, Lawrence L.J. Luntungan, Aldian Hein Macpal, Yuliana Malintoi, Adrianus Mamuaja, Jane M. Mandolang, Meilisa Mangindaan, Remy Mangindaan, Remy EP Manik, Franciskus K.I. Mantiri, Desy M. H Markus T. Lasut Mberato, Shellyn Prastisia Medy Ompi Moningkey, Rudy Natalie D Rumampuk Nowin, Edgar Pangalila, Novelia Paransa, Darus J Paulus, James Paulus, James H Paulus, James J.H. Pipih Suptijah Poluan, Gledys Giacinta Pratasik, Silvester R. T. D. Maramis Ramadan, Febrian Reiny A. Tumbol Rember, Unstain NWJ Rimper, Joice R. T. S. L . Rimper, Joice RTSL Rizal W Suleman Robert A. Bara Roeroe, Kakaskasen A Roeroe, Kakaskasen A. Rumampuk, Detty N Rumampuk, Natalie D T Rumampuk, Natalie D.C Salaki, Meiske Sandra Tilaar Senduk, Anastasia V Silvana Dinaintang Harikedua Sinjal, Chatrien AC Sondak, Calvyn Sondak, Calvyn F.A Sondakh, Calvyn A. Stenly Wullur Sumual, Sarah S. Suzanne L Undap Tampi, Kezia Keren Erika Tarigan, Frista Ukar, Melania Undap, Suzanne J Wilhelmina Patty Wilmy E Pelle Winda Mingkid Wondal, Bella